American Society of Hematology

Figure 1.

$Figure 1. Evidence of eNOS protein in human RBCs. eNOS was detected in RBCs from healthy volunteers using immunofluorescence confocal microscopy (A-B), standard thin-section and immunogold cryosection (C), and freeze-fracture electron microscopy (D). (A-B) Every single RBC stains positive for eNOS (FITC labeled, green; A), shown via double-staining with an RBC-specific antibody (glycophorin A, PE labeled, red; B; n = 3). (C-D) Higher spatial-resolution confirms these results and labels eNOS in the cytoplasm and in the plasma membrane with 2 independent preparation techniques (n = 5). The concentration of eNOS label is lower in the cytoplasm than in the P-face of the plasma membrane. Bars indicate (C and inset) 1 μm; (D) 0.2 μm.$

Evidence of eNOS protein in human RBCs. eNOS was detected in RBCs from healthy volunteers using immunofluorescence confocal microscopy (A-B), standard thin-section and immunogold cryosection (C), and freeze-fracture electron microscopy (D). (A-B) Every single RBC stains positive for eNOS (FITC labeled, green; A), shown via double-staining with an RBC-specific antibody (glycophorin A, PE labeled, red; B; n = 3). (C-D) Higher spatial-resolution confirms these results and labels eNOS in the cytoplasm and in the plasma membrane with 2 independent preparation techniques (n = 5). The concentration of eNOS label is lower in the cytoplasm than in the P-face of the plasma membrane. Bars indicate (C and inset) 1 μm; (D) 0.2 μm.

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