American Society of Hematology

Figure 2.

$Figure 2. Subcellular localization of NOS protein in RBCs. (A) Stomatin label is found exclusively on the P-face of the plasma membrane. No label is seen on the E-face or the cytoplasm. The arrows indicate stomatin label over cross-fractured P-face. (B) Fracture of a single RBC permits simultaneous viewing of portions of the plasma membrane (P-face) and the cytoplasm. Gold markers of distinct sizes were used to identify eNOS (18-nm gold) and stomatin (12-nm gold). Both are present on the P-face of the plasma membrane, whereas merely eNOS is found in the cytoplasm. Arrowheads indicate the line of fracture plane between the plasma membrane and the cytoplasm. (C) In contrast to the positive staining in the cytoplasm and the P-face of the RBC membrane, neither eNOS label nor stomatin label is found on the E-face of the plasma membrane. Bars, 0.2 μm.$

Subcellular localization of NOS protein in RBCs. (A) Stomatin label is found exclusively on the P-face of the plasma membrane. No label is seen on the E-face or the cytoplasm. The arrows indicate stomatin label over cross-fractured P-face. (B) Fracture of a single RBC permits simultaneous viewing of portions of the plasma membrane (P-face) and the cytoplasm. Gold markers of distinct sizes were used to identify eNOS (18-nm gold) and stomatin (12-nm gold). Both are present on the P-face of the plasma membrane, whereas merely eNOS is found in the cytoplasm. Arrowheads indicate the line of fracture plane between the plasma membrane and the cytoplasm. (C) In contrast to the positive staining in the cytoplasm and the P-face of the RBC membrane, neither eNOS label nor stomatin label is found on the E-face of the plasma membrane. Bars, 0.2 μm.

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