Figure 3.
![Figure 3. Evidence of eNOS expression in RBCs. (A) Western blot analysis revealed an eNOS-specific band at 140 kDa in preparations of RBCs (lanes 2 and 3 are derived from diluted preparations [Hct 20%, 10%, and 5%, respectively; with 15, 7.5, and 3.8 μg protein/lane]). This band is equivalent to positive controls of human aortic endothelial cells (HAECs; 2 μg protein/line) and myocardium (cell lysate; 1 μg protein/line). (B) The 450-bp fragment of eNOS mRNA from RBCs corresponds to the control of HUVECs and whole blood. The purity of the RBC fraction was confirmed by mRNA amplification of reference proteins: endoglin for HUVECs, β-globin for RBCs, β-3 integrin for platelets, and CD45 for WBCs. Whole blood was taken as positive control.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/7/10.1182_blood-2005-10-3992/5/zh80070693530003.jpeg?Expires=1735843218&Signature=UVJblcLYn9eoPAy0XBztCLvzuOPERirRnQ~hx2JzzqgBgfTThf9-6nfxFfWAD8DwIwwGuMTZ-URgOXX~I8LaC-DbjpnWpeYD3thTwNoydsy2-syvucoDNITXJ7mbx9Cx3y31tOxhJa2D0mM8H5KjUznUGIoULmh0mGMqnPbLWwhz1hlH3U-wtOiMl8gBQOBAoPLytMKlCPLQaHoZT7V6GVUSX4ovChSuDDeUHycbYDvXRRxyd8IubT1GjNUT1i~hHz3~Sq0d-eriSTPmEQ8yoR9uIzShEhtKySE-bUuJfP6s1zxf3DF41GiVJhSDDF9yCgUWN0OYbCwGIk1axNBR1A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Evidence of eNOS expression in RBCs. (A) Western blot analysis revealed an eNOS-specific band at 140 kDa in preparations of RBCs (lanes 2 and 3 are derived from diluted preparations [Hct 20%, 10%, and 5%, respectively; with 15, 7.5, and 3.8 μg protein/lane]). This band is equivalent to positive controls of human aortic endothelial cells (HAECs; 2 μg protein/line) and myocardium (cell lysate; 1 μg protein/line). (B) The 450-bp fragment of eNOS mRNA from RBCs corresponds to the control of HUVECs and whole blood. The purity of the RBC fraction was confirmed by mRNA amplification of reference proteins: endoglin for HUVECs, β-globin for RBCs, β-3 integrin for platelets, and CD45 for WBCs. Whole blood was taken as positive control.
