Strong mutagenic potential of TMZ in vivo. (A) Mutation frequency of BM cells derived from animals treated with either CP or TMZ in vivo, measured with the small blue mouse mutation indicator strain. (B) Frequency of point mutations (PM) in BM cells derived from animals treated with either CP or TMZ in vivo. Values shown are mean ± 1 SEM; n = 18 for untreated/vehicle, n = 4 for CP, and n = 4 for CP. ^*P < .05.