Loss of macrophages in c-FLIP^f/f LysM-Cre mice. (A) Representative c-FLIP^f/f and c-FLIP^f/f LysM-Cre mice show the decreased size observed in c-FLIP^f/f LysM-Cre mice. (B) Body weight of c-FLIP^f/f and c-FLIP^f/f LysM-Cre mice at 6-8 weeks of age. Black bars represent c-FLIP^f/f mice and white bars represent c-FLIP^f/f LysM-Cre mice. Data were obtained in 4 independent experiments (n = 7-9; ***P < .001). (C) Wild-type, floxed, and deleted c-FLIP alleles in BMDM from c-FLIP^f/+, c-FLIP^f/f, c-FLIP^f/+ LysM-Cre, or c-FLIP^f/f LysM-Cre mice as detected by Southern blot. Film was scanned with a Canon CanoScan n122ou scanner. (D) Cytospins of Day 3 thioglycollate-elicited PEC samples from c-FLIP^f/f (left) and c-FLIP^f/f LysM-Cre mice (right). Original magnification 200×. Images were obtained using a Zeiss Axiovert 200 (20×/0.20 NA objective lens) with an AxioCam MRC camera and AxioVision Rel. 4.8 software. (E) Quantification of macrophages, neutrophils, and monocytes in Day 3 thioglycollate-elicited PEC. Data were obtained in 4 independent experiments (n = 5-6; NS, not significant; ***P < .001). (F) Absolute numbers of CD4⁺ or CD8⁺ T cells, B220⁺ B cells, CD11b⁺Ly6C^int neutrophils, SSC^loCD11b⁺Ly6C^hi inflammatory monocytes, and SSC^lo CD11b⁺ Ly6C^lo resident monocytes in peripheral blood as determined by flow cytometry. Data were obtained in 7 independent experiments. n = 8-10; NS, not significant; P < .05; **P < .01; ***P< .001. Error bars represent standard deviations.