Bone marrow stromal macrophages are lost and hematopoiesis is altered in c-FLIP^f/f LysM-Cre mice. (A) Total bone marrow cellularity and absolute numbers of F4/80⁺, F4/80⁺ CD11b^hi, and F4/80⁺ CD11b^lo bone marrow macrophage populations as determined by flow cytometry. Data were obtained in 6 independent experiments (n = 7-11; NS, not significant; *P < .05; **P < .01; ***P < .001). (B) Absolute numbers of B220⁺ B cells and CD11b⁺ Gr1⁺ neutrophils in bone marrow of c-FLIP^f/f and c-FLIP^f/f LysM-Cre mice as determined by flow cytometry. Data were obtained in 3 independent experiments (n = 4-7; ***P < .001). (C) Representative bone marrow cytospins from c-FLIP^f/f (left) and c-FLIP^f/f LysM-Cre (right) mice showing immature (black arrowheads) and mature neutrophils (red arrowheads), lymphocytes (green arrowheads), monocytes (orange arrowhead), and macrophages (blue arrowhead). Original magnification 200×. Images were obtained using a Zeiss Axiovert 200 (20×/0.30 NA objective lens) with an AxioCam MRC camera and AxioVision Rel 4.8 software. (D-F) Absolute numbers of progenitor cells in bone marrow as determined by flow cytometry. Data were obtained in 2 independent experiments. n = 4; NS, not significant; **P < .01; ***P < .001. (D) Absolute numbers of Lin⁻ (CD3ϵ⁻CD4⁻CD8⁻CD11b⁻B220⁻) cells. (E) Absolute numbers of LSK (Lin⁻Sca-1⁺c-Kit⁺) cells, CMP (Lin⁻c-Kit⁺Sca-1⁻ CD34⁺ FcγR^lo), and GMP (Lin⁻c-Kit⁺Sca-1⁻ CD34⁺ FcγR^hi) populations. (F) Absolute numbers of MEP (Lin⁻c-Kit⁺Sca-1⁻ CD34⁻ FcγR^lo) cells. In all panels, black bars represent c-FLIP^f/f mice and white bars represent c-FLIP^f/f LysM-Cre mice. *P < .05; **P< .01; ***P < .001. All error bars represent standard deviations.