Neutrophil clearance is delayed in c-FLIP^f/f LysM-Cre mice. (A) Kinetics of neutrophil production and clearance in c-FLIP^f/f and c-FLIP^f/f LysM-Cre mice. Mice were injected intaperitoneal with BrdU. Blood samples were taken at time points from 24-192 hours postinjection, and the absolute number of BrdU⁺ neutrophils was quantified by flow cytometry (top). Bottom, absolute number of BrdU⁺ neutrophils normalized to the maximum number of BrdU⁺ neutrophils observed in each mouse strain. Data were obtained in 3 independent experiments. Triplicate values from a single experiment (n = 3-6). (B) Accumulation of annexin V⁺ 7-AAD⁺ neutrophils in the spleens of c-FLIP^f/f LysM-Cre mice. Data were obtained in 2 independent experiments (n = 6-7). (C) Levels of lactate dehydrogenase in plasma of c-FLIP^f/f and c-FLIP^f/f LysM-Cre mice. (n = 6; ** P < .01) In all panels, black bars or boxes represent c-FLIP^f/f mice and white bars or boxes represent c-FLIP^f/f LysM-Cre mice. Error bars represent standard deviations.