Neutrophilia, splenomegaly, decreased body size, and increased G-CSF production are secondary to the loss of macrophages in c-FLIP^f/f LysM-Cre mice. (A) c-FLIP^f/f recipient mice were lethally irradiated before transfer of congenically marked bone marrow. Transferred bone marrow was either c-FLIP^f/f, c-FLIP^f/f LysM-Cre, or a 1:1 mixture of c-FLIP^f/f and c-FLIP^f/f LysM-Cre. (B-G) Posttransfer analysis of bone marrow chimeric mice. Wild-type (WT) chimeric mice are represented by black boxes, knock-out (KO) chimeric mice are represented by white boxes, and mixed chimeric mice are represented by gray boxes. In mixed chimeric mice, cells of WT donor origin are represented by black boxes and cells of KO donor origin are represented by white boxes. Data in B-F were obtained in a single experiment (NS, not significant; *P < .05; **P < .01; ***P < .001). (B) Absolute numbers of thioglycollate-elicited PEC macrophages. Recipient mice were injected intaperitoneally with thioglycollate. PEC samples were harvested 3 days after injection, and differential counts were performed on cytospins. (C) Absolute numbers of neutrophils in peripheral blood of recipient mice as determined by flow cytometry. (D) Absolute numbers of inflammatory monocytes in peripheral blood of recipient mice as determined by flow cytometry. (E) Body weight of recipient mice 17 weeks after transfer. (F) Spleen weight of recipient mice 17 weeks after transfer. (G) Cytokine levels in plasma of recipient mice 2 weeks after transfer. Data were obtained in a single experiment. (n = 4-5). *P < .05; **P < .01; ***P < .001. All error bars represent standard deviations.