Analysis of expression of several markers on tetramer⁺ thymocytes from wild-type and T-bet^–/– mice. (A) CD1d tetramer⁺ TcRβ⁺ thymocytes from wild-type and T-bet^–/– mice were sorted and total RNA prepared. Expression levels of IFNγ, IL-4, CD122, granzyme B, Fas ligand, CCR5, and CxCR3 between the 2 cell populations was measured by quantitative PCR using specific primers and probes. The amount of transcript was normalized to the amount of HPRT in each sample. Results are shown as mean ± standard deviation. (B) C57BL/6 and T-bet^–/– thymocytes were stained with CD1d tetramer, TcRβ, and diverse markers. CD1d tetramer⁺ TcRβ⁺ cells were gated and analyzed for the expression of CD38, NKG2D, 2B4, CD94, Ly6C, and CD122. Percentages of positive cells are indicated.