Surface marker expression and cytokine secretion by DCs stimulated with β-glucans. (A) BMDCs from WT, CD11b^−/−, or dectin-1^−/− mice were stimulated with particulate β-glucan (100 μg/mL) and surface marker expression was assessed by flow cytometry. One representative histogram from 3 independent experiments with similar results. (B) BMDCs were stimulated with soluble PGG β-glucan and surface marker expression was measured by flow cytometry. (C) Supernatants from particulate β-glucan–stimulated DCs were collected and assayed for TNF-α, IL-12, IL-6, and IL-10 (**P < .01; ***P < .001).