CD4 T-cell differentiation and CD8 priming and differentiation stimulated by β-glucan–activated DCs. (A) OVA Tg CD4⁺ T cells were cocultured with BMDCs from WT, CD11b^−/− or dectin-1^−/− mice in the presence or absence of OVA and β-glucans. CD4⁺ T cells cultured with β-glucans were used as controls. Percent of CD4⁺IFN-γ⁺ cells and CD4⁺ IL-17⁺ cells is shown (n = 4). (B) CFSE-labeled OVA Tg CD8⁺ T cells were cultured together with BMDCs in the presence of OVA and β-glucan. CD8⁺ T cells cultured with β-glucans were used as controls. Graphs show CFSE dilution versus intracellular IFN-γ on day 3 of culture. Percent of CD8⁺IFN-γ⁺ cells is shown (n = 4).