Particulate β-glucan treatment significantly reduces tumor burden with enhanced antitumor immunity. (A) Groups of WT mice were implanted subcutaneously with EO771/OVA tumor cells. After palpable tumors formed, mice were treated daily with or without particulate β-glucan for 2 weeks. Tumor diameter was recorded at the indicated time. Tumor mass was weighted when mice were killed. (B) Single cell suspensions prepared from tumor samples or spleen as indicated were stained with fluorochrome labeled mAbs. Summarized data are shown. (C-D) Single cell suspensions from tumors were stimulated with PMA plus ionomycin and stained intracellular IFN-γ and IL-17. Cells were gated on CD4⁺ (C) or CD8⁺ (D) T cells. (E) Single cell suspensions from tumors or tumor DLNs were stimulated with ovalbumin and intracellular IFN-γ staining was performed. (F) Splenocytes were labeled with CFSE and then stimulated with ovalbumin. Graphs show CFSE dilution versus intracellular IFN-γ production. (G). RNAs from tumor specimens were extracted and qRT-PCR was performed for the indicated cytokines and arginase.