Alterations in the expression of IL-7Rα, GABPα, and GFI1 mRNA in naive and EM_CD45RA+ IL-7Rα^high CD8⁺ T cells in response to cell stimulation. PBMCs from healthy young subjects were stained with antibodies to CD8, CD45RA, CCR7, and IL-7Rα and were sorted into naive and EM_CD45RA+ IL-7Rα^high CD8⁺ T cells. The expression of IL-7Rα, GABPα, and GFI1 mRNA in freshly isolated (0 hour), 24-hour PBS- and PHA-incubated naive (A) and EM_CD45RA+ IL-7Rα^high (B) cells was measured using real-time PCR. Graphs show the mean expression (n = 2) and standard deviation (SD) of the indicated genes. Data were normalized to β-actin expression in individual samples.