Figure 1.
Anion-exchange chromatography of placental extract. Papain-digested and cetylpyridinium chloride/ethanol–precipitated placental extract was applied to a Mono Q column and eluted with NaCl in Tris buffer, pH 7.4, as shown. The amount of GAG in each fraction was determined by the carbazole assay for uronic acid. Fractions were pooled as follows: peak I, fraction 2; peak II, fractions 14-32; peak III, fractions 34-39.