Agarose gel analysis of Mono Q peak III. (A) Samples of GAG standards (15 μg/well) or desalted Mono Q peak III (50 μg/well) were electrophoresed on a 0.5% agarose gel for 1 hour. Subsequently, the gel was stained with 0.1% toluidine blue. (B) Material from peak III was treated sequentially with nitrous acid, chondroitin AC II–lyase, and chondroitin ABC–lyase. A sample from each step was subjected to electrophoresis. HS indicates heparan sulfate; DS, dermatan sulfate; and CS, chondroitin 6-sulfate.