TIP110 expression in phenotyped cord blood CD34⁺ cells and mouse bone marrow HSCs and HPCs. (A) Freshly isolated CD34⁺ cells were cultured in IMDM medium supplied with FBS and SFT for 5 hours and sorted into CD34⁺CD38⁻, CD34⁺CD38⁺, CD34⁺CD133⁺, CD14⁺, CD15⁺, and CD36⁺ subpopulations. TIP110 expression in each of these subpopulations was determined by semiquantitative RT-PCR and Western blotting (WB). (B-C) Freshly isolated CD34⁺ cells were cultured in IMDM medium supplemented with FBS and SFT, and harvested at days 0, 1, 6, and 10 to assess TIP110 expression by RT-PCR, Western blotting (B), and flow cytometry using anti-CD34 antibody staining (C). Results shown in panels A-C are from 1 experiment representative of 3 separate reproducible experiments. (D) Tip100 mRNA expression, as assessed by semiquantitative RT-PCR, in purified populations of HSCs (CD34⁻LSK, CD34⁺LSK, and LSK cells) and HPCs (MEPs, GMPs, and CMPs) compared with bone marrow (BM) and peripheral blood (PB).