Effects of TIP110 haploinsufficiency on HPCs. (A) Diagram of TIP110 conditional knockout cassette consisting of 3 TIP110 genomic loci: exons 9-12, 13-18, and 19, 3 loxP sites in between, and a neo selection marker. Breeding of TIP110 conditional knockout animals with hematopoietic linage-promoter Tie2-driven Cre^TG mice led to TIP110 haploinsufficiency (+/−) in hematopoietic organs. (B) Bone marrow and spleen cells were harvested from age-matched C57B/L6 and TIP110^+/− mice to determine TIP110 expression by Western blotting. (C-D) Cells were isolated from bone marrow and spleens of age-matched C57B/L6 and TIP110^+/− mice and assayed for absolute numbers (C) and cycling status (D) of HPCs, as described in the legend to Figure 2. Open bars show WT cells; closed bars, TIP110^+/− cells. Data shown in panel B are from 1 of 3 reproducible experiments. Data in panels C and D are expressed as means ± SEM of 14 individually assessed mice for each group from a total of 3 independent experiments.