Figure 7
Figure 7. Reciprocal regulation of TIP110 and CMYC and GATA2 regulation of TIP110. (A-B) 293T cells were transfected with pGL3.CMYCProm-Luc and increasing amounts of TIP110 expression plasmid (A) or shTIP110 plasmid (B). 293T cells were transfected with pGL3.TIP110Prom-Luc and increasing amounts of CMYC expression plasmid (C) or shCMYC plasmid (D), and increasing amounts of GATA2 expression plasmid (E) or shGATA2 plasmid (F). Transfected cells were cultured for 48 hours and then harvested for luciferase reporter gene assay. In all transfections, either pc.DNA3 or shCtrl, as indicated, was included to equalize the total amount of transfected DNA in each experiment, and the pTK-βgal plasmid was included to normalize the transfection efficiency variations among all transfections. Data represent the means ± SEM of 3 representative experiments, each done in triplicate.

Reciprocal regulation of TIP110 and CMYC and GATA2 regulation of TIP110. (A-B) 293T cells were transfected with pGL3.CMYCProm-Luc and increasing amounts of TIP110 expression plasmid (A) or shTIP110 plasmid (B). 293T cells were transfected with pGL3.TIP110Prom-Luc and increasing amounts of CMYC expression plasmid (C) or shCMYC plasmid (D), and increasing amounts of GATA2 expression plasmid (E) or shGATA2 plasmid (F). Transfected cells were cultured for 48 hours and then harvested for luciferase reporter gene assay. In all transfections, either pc.DNA3 or shCtrl, as indicated, was included to equalize the total amount of transfected DNA in each experiment, and the pTK-βgal plasmid was included to normalize the transfection efficiency variations among all transfections. Data represent the means ± SEM of 3 representative experiments, each done in triplicate.

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