Angptl3 supports HSC repopulation in vivo. (A, B) WT donor BM CD45.1 cells (5 × 105 cells) were transplanted into lethally irradiated CD45.2 Angptl3-null or WT recipient mice. The frequencies and numbers of donor Lin−Sca-1+Kit+Flk2−CD34− cells in the BM of the transplanted mice at 4 months after transplantation are shown in panel A and panel B, respectively (*P < .05, n = 5). (C) WT donor BM CD45.1 cells (5 × 105 cells) were transplanted into lethally irradiated CD45.2 Angptl3-null or WT recipient mice. These primary transplanted mice were killed at 2 months after transplantation and CD45.1 BM cells were collected and pooled. One million of pooled BM cells, along with 105 freshly isolated CD45.2 competitor cells, were injected into each of 5 lethally irradiated CD45.2 secondary recipient mice. The mice were then analyzed for the engraftment of original donor CD45.1 cells at 4-24 weeks after transplantation (*P < .05, n = 5). (D) Multilineage contribution of donor cells in secondary transplanted recipients at 24 weeks after transplantation (n = 5).
