Transcription factor expression pattern in CD11b⁺ MHCII^high CDC-like cells generated from CCR9⁻ PDCs. CCR9⁺ and CCR9⁻ PDCs sorted from primary BM cells were incubated with medium alone or 50% IEC-SN for 48 hours. Relative mRNA expression of transcription factors was measured by quantitative RT-PCR in sorted BST2^low IEC-SN DCs and BST2^high IEC-SN PDCs generated from CCR9⁻ PDCs cultured with IEC-SN as well as in sorted BST2^high PDCs generated from CCR9⁺ PDCs cultured with medium alone. The expression patterns of transcription factors in splenic CD8α⁺ and CD8α⁻ DCs are shown for comparison. Results of one representative of 2 experiments are shown.