Expanded CIK cells from lymphoma patients present comparable cytotoxicity against autologous or allogeneic freshly isolated lymphoma cells. (A) CIK cultures from healthy normal donors were used as effectors. The patient-derived purified lymphoma cells were labeled with calcein-AM and incubated 4 hours with CIK cells at effector-to-target ratios of 30:1, 10:1, and 5:1. The mean percentage of specific lysis ± SD from 3 independent experiments is shown. (B) CIK cells were expanded from PBMCs from 2 patients (with Waldenström macroglobulinemia and follicular lymphoma, respectively) and were used as effector cells against their own purified blasts as targets (patient1 target, left panel; patient 2 target, right panel), testing them in both the autologous combination (CIK1 vs patient 1 or CIK2 vs patient 2, black columns) or allogeneic combination (CIK1 vs patient 2 and CIK2 vs patient 1, gray columns), at the indicated effector-to-target ratios. Data are expressed as mean percentage ± SD of 3 independent experiments.