Figure 2
Plasminogen activation rates over a range of tPA concentrations in the presence of fibrin formed at high and low thrombin concentrations. Clots were prepared using thrombin at 100nM fibrin100 (◇, solid line) or 10nM fibrin10 (○, dashed line) and subsequently tPA from 0.15 to 1.2nM overlaid in the presence of S-2251. Initial rates were measured up to an absorbance change of 0.1. Data are plotted as log dose tPA (nM added to the clot) versus log response (rate of change of absorbance/s2 × 109).
