Validation of DC miR profile by RT-PCR. (A) Computational prediction of miRs targeting IL-6 3′UTR by the miRanda, MicroCosm Target, and PITA Top programs The miR-142-3p and miR-223 were predicted by all 3 programs. (B) The potential miR target sequences in the 3′UTR of IL-6 mRNA that are computationally predicted in DCs are shown and the seed sequence pairings are indicated by the lines. (C) Validation of miR expression pattern at baseline and the response to LPS. DCs were treated with either LPS or diluent for 12 hours and analyzed. The levels of miRNA-142-3p, miR-223, Let-7a, miR-27a/b, miR-155, and miR-146a were analyzed by TaqMan quantitative RT-PCR. Expression of miRNAs is presented relative to snoRNA135. Data are representative of 2 to 6 separate experiments with similar results (mean ± SEM).