TLR2 increases polyploidization in mouse megakaryocytes. WT (A) and TLR2^−/− (B) mouse bone marrow was treated for 3 days with 50 ng/mL mTPO or 1μg/mL Pam3CSK4 (PAM). (C) WT mouse bone marrow was treated for 3 days with 1 μg/mL of PAM, 50μM Bay 11-7082, 50μM LY294002, 50μM U0126, or DMSO. The percentage of mouse megakaryocytes at each level of DNA content was determined by flow cytometry (n = 3). *P < .05; **P < .01; ***P < .001 compared with NT; ^αP < .05, ^ααP < .01; and ^αααP < .001 comparing mTPO with PAM (A-B) or with inhibitor alone (C).