Pam3CSK4 treatment in WT mice affects platelet counts and megakaryocyte maturation. WT and TLR2^−/− mice were injected once intraperitoneally with saline or 4 mg/kg of Pam3CSK4, and monitored for 1 week. (A) Platelet counts were done on blood samples from each mouse at days 3, 5, and 7 after the injection. Each point is the average ± SD of 3 mice. (B-C) Megakaryocyte ploidy determined on days 3, 5, and 7 (n = 3). *P < .05, **P < .01, and ***P < .001 compared with saline treatment on that day.