Doxorubicin and carboplatin treatment of wild-type mice. Eight-week-old C57BL6/J, 129/SvEvTac, and BALBc/J mice were treated with or without 0.25 mg doxorubicin or 2.5 mg carboplatin and then harvested 3 days later. Mice were analyzed for reticulocyte counts (A), serum iron (Fe) concentrations (B), Tf saturations (C), spleen masses (D), hemoglobin levels (E), and liver hepcidin levels relative to β-actin levels as measured by QPCR (F). Each value represents 5 mice. A ‘*’ above a bar indicates that the value differs significantly relative to untreated mice of the same strain (t test, P < .05).