Doxorubicin treatment of wild-type and hpx mice. Eight-week-old wild-type (wt) and hpx mice were injected intraperitoneally with 0.25 mg doxorubicin and harvested and analyzed at 0, 3, 6 and 8 days for reticulocyte counts (A), spleen masses (B), hemoglobin levels (C), serum iron (Fe) concentrations (D), total iron-binding capacities (E), and liver hepcidin levels relative to β-actin levels as measured by QPCR (F). Each point represents 4 to 6 mice. The symbols ‘#’ and ‘*’ indicate statistical significance as in Figure 1.