Further analysis of the effect of chemotherapeutic agents on hpx mice. (A-B) Splenic Gdf15 (A) and Twsg1 (B) levels were measured by QPCR and expressed as a ratio to β-actin levels in wild-type (wt) mice, untreated hpx mice (–), or hpx mice treated with 1.5 mg human Tf (TF) or 0.5 mL wild-type mouse serum (serum) every other day for 3 weeks or 0.25 mg doxorubicin (dox) for 6 days. Each bar represents 3 to 6 mice. Black and gray asterisks indicate statistical significance between the labeled value and wild-type or hpx mouse value, respectively. (C) Hepatic Bmp6 levels relative to β-actin levels were measured by QPCR in samples from doxorubicin-treated wild-type and hpx mice from Figure 3. The symbols ‘#’ and ‘*’ indicate statistical significance as in Figure 1. (D) Hepatic Bmp6 levels relative to β-actin levels were measured by QPCR in samples from wild-type mice treated with doxorubicin or carboplatin from Figure 2. A ‘*’ indicates statistical significance relative to untreated mice as in Figure 2.