Doxorubicin and Tf treatment of hpx mice. Eight-week-old hpx mice were treated with or without 0.25 mg doxorubicin (+dox and -dox) on day 0; mice were then treated with or without 10 mg human Tf (TF) or 2.5 mg mouse Tf (Tf) on day 3 and 5. Mice were then harvested on day 6 and analyzed for reticulocyte counts (A), spleen masses (B), hemoglobin levels (C), serum iron (Fe) levels (D), Tf saturations (E), and hepcidin levels relative to β-actin levels by QPCR (F). Untreated wild-type (wt) mice, indicated in parentheses, were included for reference. Each bar represents 4 to 5 mice. Numbers indicate outcome of t test between bracketed values.