Induction of PDI in AML patients suppresses CEBPA protein. (A) Real-time PCR of PDI mRNA levels in AML patients according to the FAB classification. Mature granulocytes and monocytes from 6 healthy donors are indicated for comparison. Error bars indicate SD. (B) Real-time PCR results of leukemic cells from 4 AML patients for CEBPA and PDI mRNAs. The patients had AML-M2 (#1), -M0 (#2), -M5 (#3), and -M4 (#4). (C) Western blot analysis using whole-cell lysates from blasts of the same 4 AML patients. Membranes were stained with Abs against CEBPA, PDI, and calreticulin. β-actin was used as loading control.