Characterization of the ZNF112 cell line. (A) Fluorescence microscopy shows that all ZNF112 cells are GFP⁺ with stable expression (Western blot) of the ZMYM2-FGFR1 fusion protein. The murine pro-B-cell line BaF3 expressing ZMYM2-FGFR1 (BaF3-ZNF) was used as a positive control, and mesenteric lymph node cells (MLN) provide the negative control. (B) CGH array analysis of ZNF112 cells shows additional copies of chromosomes (Chr) 7, 10, 15, and loss of regions within 16qA1 and 14qC2. (C) Spectral karyotyping analysis confirmed the aCGH profiles for ZNF112 cells and also shows loss of the Y chromosome and an additional unidentified marker chromosome (M). (D) FISH using RP23 BAC clones identifies the 16;Y translocation (see text). (E) Leukemias resulting from syngeneic grafts of ZNF112 cells into mice without irradiation show high numbers of white blood cells that are Gr1⁺ and Mac1⁺ (F). Mice with ZNF112 grafts show hepatosplenomegaly and enlarged lymph nodes with GFP⁺ cells.