Aberrant distribution of the MLL protein at MLL fusion target gene loci. A 500-bp sliding window was used to scan the region spanning −20 kb to 100 kb from transcription start site (TSS) for each gene. Binding signals within each window were averaged for the respective set of genes. (A) MLL binding curve was plotted for each of the 5 cell lines when all MLL protein target genes (wild-type and fusion) were considered. Arrows point to MLL binding curves obtained from 3 MLL-rearranged cell lines with ML-2 cells on the top. (B) Average MLL binding curve for “24 MLL-AF6 target genes” (Figure 2B) were considered. Solid arrows point to MLL binding curves obtained from 3 MLL-rearranged cell lines; broken arrows 2 MLL nonrearranged cell lines. (C) All MLL protein targets excluding “24 MLL-AF6 target genes” (Figure 2B) were considered. (D) Average signals of non-MLL target genes for each cell line were plotted as negative controls.