Stimulation of T cells with NKG2D ligand–positive tumor cells activates β-catenin and decreases anti-inflammatory genes. (A) chNKG2D (closed bars) and wtNKG2D (open bars) T cells were cultured with media, P815, P815-MICA, or U937 cells in the presence of anti-CD3 Abs or isotype (iso) control Abs. After 5 hours, β-catenin was measured by staining cells with anti–β-catenin Abs. (B) chNKG2D T cells and wtNKG2D T cells cultured with P815 (open bars) or P815-MICA (closed bars) cells in the presence of anti-CD3 Abs or isotype control Abs. After 8 hours, gene expression was determined by RT-PCR. Data are shown as the fold change in gene expression compared with T cells cultured with P815 cells for each Ab. Data shown were obtained from 1 donor and are representative of data from 3 donors. Stimulation of T cells with P815-MICA cells significantly changed gene expression compared with stimulation with P815 cells (**P < .01 and ***P < .001).