Inhibition of IFN-γ and GH signaling pathways by CK2 inhibitors. (A-C) Cell lysates were immunoblotted with the indicated antibodies. (A) MEFs were pretreated with TBB (50μM) or emodin (50μM) for 2 hours and then stimulated with 5 ng/mL of IFN-γ for 30 minutes. The densitometric ratios of P-Y-STAT-1 versus STAT-1 were calculated. The values of lanes 3 and 4 were compared with that of lane 2 (control, no inhibition) and the percentage of inhibition was determined. (B) MEFs were pretreated with TBB (50μM) or emodin (50μM) for 2 hours and then stimulated with 5 ng/mL of IFN-γ for 30 minutes. (C) γ2A-GHR-JAK2 cells were pretreated with TBB (50μM) or emodin (50μM) for 2 hours and then stimulated with 250 ng/mL of GH for 10 minutes. The densitometric ratios of P-Y-STAT-5 versus ACTIN were calculated. The values of lanes 3 and 4 were compared with that of lane 2 (control, no inhibition) and the percentage of inhibition was determined.