Effect of cytokines on CD94/NKG2A expression. (A-C) A total of 0.5 to 1 × 10⁵ naive CD8⁺ T cells purified from 2C TCR Tg mice (A-C) or from WT (Balb/c) and STAT6^−/− mice (D) were stimulated with either plate-bound anti-CD3 monoclonal antibody (A, 2.5 μg/mL; C-D, 5 μg/mL), PMA plus ionomycin (PMA/Iono; B), or 5 × 10⁵ irradiated T-depleted B6 (H-2^b) spleen cells pulsed with 10μM SIYR peptide (sAPC-SIYRp; B) in the presence or absence of the indicated cytokines at various concentrations (A-B,D, all 50 ng/mL except IFN-β used at 2 × 10² units/mL; C, 0-100 ng/mL). On day 2 after culture, cells were analyzed for CD94 expression by flow cytometry. The percentages of cells in the respective regions (C) are indicated. Data (A-D) are representative of 3 or 4 independent experiments (A-B,D; mean ± SD).