Interaction of endogenous Bcl-2 with Rac1 in primary tumor tissue. (A) Table representing the different patient samples used in the analysis. (B) Whole cell lysates (WCL) from primary tissue derived from patient 1 (B-cell lymphoma), PBLs from healthy donor, IMR90 human fibroblasts, rat L6 smooth muscle cell fibroblasts, and mouse NIH3T3 fibroblasts were analyzed for the expression of Rac1 and Bcl-2 (C-2, Santa Cruz Biotechnology). (C) Cell lysates were immunoprecipitated with anti-Rac1 and probed with anti–Bcl-2 and anti-Rac1 (as internal control). (D) Cell lysates from clinical material (patients 2-5) and PBLs were immunoprecipitated with anti-Rac1 and probed with anti–Bcl-2 using Western blot analysis. (E) Cell lysates from PBLs from 3 healthy donors were immunoprecipitated with anti–Bcl-2 and probed with anti-Rac1.