pDCs from DKO-hu mice express both HIV coreceptors, and are productively infected by HIV-1 in vivo. (A) Total BM cells or splenocytes were analyzed by FACS. CD3⁻CD4⁺CD123⁺ pDCs were further analyzed for CCR5 and CXCR4 expression. Dotted gray lines represent IgG isotype control. (B,C) At 8 days after HIV infection (R3A virus), splenocytes (B) and BM cells (C) were stained with surface markers, followed by HIV p24 intracellular staining. The numbers in the plots represent p24⁺ percentage of CD3⁺ T cells and CD3⁻BDCA2⁺CD123⁺ pDCs. Samples from 1 mock and 3 HIV-infected DKO-hu mice are shown. (D) BM cells from HIV-R3A-HAS–infected DKO-hu mice were analyzed by flow cytometry. The percentage of HSA (mouse CD24) expression on CD3⁺CD8⁻ T cells and CD3⁻CD123⁺ pDCs from 1 mock and 2 HIV infected DKO-hu mice are shown.