Intracellular pools of band 3, RhAG, and Rh are visible early in erythropoiesis. The top panel represents cells removed from cultures at the indicated time points during differentiation and cytospin and stained with May-Grünwald-Giemsa. Scale bar represents 10 μm. For immunofluorescence, cells were fixed in 0.5% acrolein, cytospun onto Cell-Tak–coated coverslips, and permeabilized using 0.05% Triton X-100. Cells were stained with antibodies against band 3 (BRIC170), RhAG (LA1818), and Rh (BRIC69) as indicated and visualized using goat anti–mouse-Alexa 488. Nuclei were stained with 4′,6-diamidino-2-phenylindole. Scale bar represents 5 μm. T = time in hours.