SR-A and MARCO regulate leukocyte trafficking during NM-induced peritonitis. WT, SR-A−/−, MARCO−/−, and DKO mice were injected intraperitoneally with 1 × 105 CFU of inactivated NM or equal volumes of PBS. After 2 hours, the percentage of resident macrophages (A), recruited monocytes (B), and neutrophils (C) from total leukocytes were determined in peritoneal exudates by flow cytometry and compared among strains. No significant difference in the number of monocytes and neutrophils was observed among the mouse strains in the vehicle-injected groups. Similarly, no significant difference was observed in the number of resident macrophages in PBS-treated SR-A and MARCO mice compared with WT mice, but PBS-treated DKO mice showed significantly fewer resident macrophages (P ≤ .01). Comparisons between PBS-treated (n ≥ 3) and NM-treated (n = 5) groups are presented and are representative of 3 independent experiments. The 2-way ANOVA with Bonferroni posttest was used to assess statistical significance. *P ≤ .05; **P ≤ .01; ***P ≤ .001
