Inflammation enhances DC migration mainly by CCR7-dependent, and moderately by CCR7-independent, mechanisms. FITC painting experiments were performed in the uninflamed or CFA-inflamed ears of WT and CCR7^−/− mice. (A) CFA injection induced a similar degree of ear swelling in WT and CCR7^−/− mice. (B) LN single-cell suspensions were analyzed by FACS for the presence of I-A/I-E⁺CD11c⁺FITC⁺ cells. The number within each plot represents the percentage of gated cells. (C) Inflammation further enhanced the CCR7 dependence of DC migration, as demonstrated by the quantification of total numbers of I-A/I-E⁺CD11c⁺FITC⁺ cells. Numbers indicate the fold difference in DC migration observed in WT compared with CCR7^−/− mice. (D) Inflammation also enhanced DC migration in CCR7^−/− mice, as evidenced by the quantification of total numbers of I-A/I-E⁺CD11c⁺FITC⁺ cells in the dLNs. **P < .01. ***P < .001.