LFA-1 activation in HSV-1–infected DCs strongly reduces their chemotaxis. The specific influence of LFA-1 and Mac-1 on the adhesion of HSV-1–infected DCs was determined by adding the anti-LFA-1 and anti-Mac-1 blocking antibodies NKI-L15 and Bear-1, respectively (both 20 μg/mL) in collagen matrices coated with fibronectin (A) or containing immobilized ICAM-1 Fc (B). In both conditions, the migration of mock-infected DCs was not significantly influenced either by LFA-1 or Mac-1 blocking antibodies. In contrast, the inhibition of LFA-1 significantly increased migration of infected DCs, whereas blocking of Mac-1 had no effect. Chemotaxis toward a CCL19 gradient was monitored by bright-field time-lapse videomicroscopy beginning 90 minutes after infection at a frame rate of 2 minutes. The average speed from 30 cells for each condition over 180 frames was calculated as the step length per minute, including nonmoving periods and displayed in micrometers per minute. Error bars represent ± SD. ***P < .001. **P < .01. *P < .05. One representative experiment of 3 is shown.