In vivo effect of γ9δ2TCR-transduced αβT cells on Daudi tumor growth. (A) Schematic overview of the experiment. RAG2^−/−γc^−/− double knockout mice received a sublethal total body irradiation with 2.0 Gy x-rays on day 0. The next day, mice were intravenously injected with 0.5 × 10⁶ Daudi-Luc cells and 10⁷ γ9δ2TCR or MDM2_81-88–specific TCR-transduced αβT cells. The same dose of T cells was injected on day 3. Mice in the indicated groups received an injection of pamidronate on day 1 IV and on days 21 and 42 IP. All mice received 6 × 10⁵ IU of IL2 with IFA subcutaneously on days 1 and 21. (B) Tumor load was measured by quantifying the luciferase activity produced by the transduced Daudi tumor cells using the Biospace In Vivo Imaging System once a week. Shown are the results of 1 representative experiment of 2 independent repetitions (n = 5).