Absence of hyperproliferation and overactivation of Unc13d^jinx/jinx;Myd88^poc/poc CD8⁺ T cells on day 12 after LCMV infection. Splenocytes from mice were stained to evaluate the percentage of CD8⁺ T cells (A) and their absolute numbers (B). Each dot represents an individual mouse. (C) Graph reporting the percentages of CD8⁺ T cells that spontaneously secrete IFNγ after 5-hour incubation in media without peptide stimulation. (D-E) IFNγ production by CD8⁺ T cells after stimulation with LCMV GP33. The percentage of IFNγ⁺ cells among the CD8⁺ T-cell population is indicated in representative dot plots (D). Absolute counts of LCMV GP33-specific IFNγ⁺CD8⁺ T cells are shown in panel E. (F) Histogram reporting the percentages of LCMV GP33-specific CD8⁺ T cells that secrete either IFNγ alone (white rectangles) or IFNγ and TNF (black rectangles). Representative data of 3 independent experiments are shown for (A-F); n ≥ 4 per group of infected mice. Error bars show SEM.