Characterization of mice carrying the Lman1 targeted allele. (A) Schematics of the cDNA structures of the WT murine Lman1 allele and the targeted allele with the gene-trap vector insertion. Locations of RT-PCR primers are indicated. (B) RT-PCR of total liver RNA isolated from Lman1^+/− and Lman1^−/− mice using 3 pairs of primers: F1-R1 indicates upstream of exons 10 and 11 junction; F2-R2, cross the exons 10 and 11 junction; and F3-R3, downstream of exons 10 and 11 junction. (C) Western blot analysis of liver lysates from WT (+/+), heterozygous (+/−), and LMAN1-deficient (−/−) mice using antibodies against LMAN1 and MCFD2. *Fusion protein of LMAN1 and β-GEO. MW indicates molecular weight.