IL-17⁺ Vγ9Vδ2 T cells promote CXCL8-mediated neutrophil migration and phagocytic activity. (A) Neutrophils were placed in the top well of a Boyden Chamber, and IL17⁺ Vγ9Vδ2 T cells were placed in the lower chamber in the presence or absence of IPP. In some experiments, neutralizing mAb to CXCL8 or isotype control mAbs were added to the lower chamber. After 3 hours of incubation at 37°C, migrated neutrophils adherent to the lower side of the membrane were stained and counted. Data are expressed as percentage of migrated cells among input. (B) Neutrophils were incubated with PE-fluorescent beads in the presence of IL17⁺ Vγ9Vδ2 T cells and IPP. In some experiments, neutralizing mAbs to CXCL8 or isotype control mAbs were added to cultures. After 2 hours, the percentage of PE⁺ neutrophils was determined FACS. Data are expressed as percentage PE⁺ neutrophils. *P < .01 and **P < .02 compared with all other groups.