Evi-1 is frequently up-regulated in leukemic cells transformed by MLL fusion protein in vivo. (A) Survival curves of sublethally irradiated recipients transplanted with BM cells transduced with either MSCV-neo-MLL-ENL (n = 11; blue) or MSCV-cMyc-ires-bcl2 (n = 12; red) are shown. (B) Expression levels of Evi-1 and HoxA9 in leukemic cells transformed by MLL fusion genes (MLL-ENL; n = 11; ■) or cMyc/bcl2 (n = 12; □) are indicated. Expression levels of Evi-1 and HoxA9 relative to BM MNCs are shown as squares. (C left) Gene structures of Evi-1a and Mds1-Evi-1 and positions of primer sets for quantitative real-time polymerase chain reaction are shown. The exons, start codons, and primers are depicted in boxes, with arrows, and with white triangles, respectively. Sequences of primers are presented in the supplemental Methods. (Right) Expression levels of Evi-1a (□) and Mds1-Evi-1 () in leukemic cells from 4 mice with high Evi-1 expression relative to BM MNCs are shown with SD.