Reporter assays using MLL-ENL deletion mutants and other oncoproteins. (A) Transactivation of pGL4-E2265 induced by MLL-AF9, AML1-ETO, PML-RARA, E2A-HLF, HoxA9/Meis1, or wild-type MLL (WT MLL) is shown. Data are presented as a relative fold increase in mean luciferase activity, with SD, after adjustment for β-galactosidase activity. (B) Schematics represent the composition of MLL-ENL deletion mutants. Numbers denote amino acid positions in wild-type MLL and ENL. Positions of CXXC DNA binding motif (red), AT-hooks DNA binding motif (AT; blue), and menin-binding motif (MBM; yellow) are shown in the schematics of intact MLL-ENL. (C) Transactivation of pGL4-E2265 induced by intact MLL-ENL or its deletion mutants is shown. Data are presented as described in (A). *P < .05 versus MLL-ENL.