Analysis of T-ALL case no.28 at diagnosis and relapse. (A) Top panel, identification of the t(1;14)(p32;q11) and a new cryptic t(7;8)(q34;q24) by R-Banding and M-FISH. M-FISH chromosome painting is shown in false color view with fluorescence profiles. Middle panel, chromosomal mapping of the t(7;8)(q34;q24) around MYC by FISH. (i) Interphase nucleus hybridized with the dual color MYC break apart fluorescence in situ hybridization probe (the 5′ probe is located immediately 5′ of MYC, and the 3′ probe ∼ 1.5 Mb 3′); (ii) metaphase FISH analysis with BAC clone RP11-440N18. The leukemic nuclei are identified by the presence of the der¹  in DAPI image; (iii) metaphase fluorescence in situ hybridization analysis with BAC clone RP11-125A17. Bottom panel, metaphase FISH mapping with BAC clone RP11-701D14 (T-cell receptorβ) and CEP8 (left image), and BAC clones RP11-701D14 and RP11-125A17 (3′ MYC; right image), confirming reciprocal fusion between T-cell receptorβ and the 3′region of MYC. (B) Array Comparative Genomic Hybridization profiles of chromosome 10 at diagnosis and relapse. The arrow indicates the anomaly at the 10q23 region. Right, a zoom on the 10q23 region is shown. Homozygous and heterozygous deletions had ratios of −1 and −4 respectively. (C) Western blot analysis of MYC and PTEN proteins at diagnosis and relapse. The MYC transcript levels normalized to ABL are indicated on the top of the blots.