Western blot analysis of PTEN and MYC in primary T-ALL samples and cell lines. (A) Western blot analysis of PTEN and MYC in 29 primary T-ALL samples. The same immunoblots were used to probe MYC, β-actin and PTEN. (B) Quantification of the relative MYC and PTEN protein amount (see “Immunoblot analysis” for details). Samples were ranked according to increasing MYC protein abundance. (C) Relative MYC transcript levels (left) and relative MYC protein levels (right) of the 29 T-ALL subset used for proteomic analysis were plotted according to NOTCH1/FBXW7 status and analyzed using nonparametric Mann-Whitney test.