Priming by DCs matured with either TLR adjuvants or the cytokine cocktail generates similar frequencies of cytokine-responsive Melan-A specific T cells. CD8⁺ T cells primed by the indicated DC population were restimulated with 10 μg/mL of Melan-A_26-35 peptide 9 days after priming, and intracellular IL2 and IFNγ were analyzed simultaneously. Bars indicate the mean proportion (± SEM) of total responding CD8⁺ T cells producing the cytokines indicated. Data are combined from 2 donors generated in independent experiments. Statistical analysis showed no significant differences between groups.